Influence and Interaction of Different Parameters on the Survival of Two Strains of Salmonella Enterica in Pig Slurry

Excretion of Salmonella enterica by late-fattening pigs may cause the dissemination of this zoonotic agent in the environment during spreading of contaminated slurry (Suraj B. Baloda et al., 2001). Slurry spreading on pastures represents a risk of contamination of animals (ruminants), and also pollution (Baudart J. et al., 2000). Even though studies have shown the purifying role of slurry (Placha I. et al. 2001), persistence of Salmonella in the soil after slurry spreading has been demonstrated (Suraj B. Baloda et al., 2001). Controlling the risk related to the spreading of this effluent entails describing the influence and interaction of parameters on the survival of Salmonella in pig slurry. A preliminary study is carried out to compare the mini-MSRV MPN technique (Fravalo P. et al., 2003) to direct isolations on brilliant green agar supplemented with rifampicin. Then experimental designs are developed using Doelhert uniform shell design (Doelhert D.H. 1971) in order to study the effect of different parameters on the survival of Salmonella enterica in pig slurry. The objective of this study is to describe the influence and interaction of different factors on the survival of two strains of Salmonella enterica in pig slurry.